Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay     

ZHANG Zhendong  ZHANG Peijun  MO Zhaolan  WANG Chunling  YU Yang 《海洋学报(英文版)》2005,24(4):155-161

Dot enzyme-linked immunosorbent assay （dot-ELISA）, indirect ELISA and Westem blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Westem blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period. From 6 to 12 h, the amount and enzyme activity of protease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5-6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards （＂marker＂） so that the alterations ofprotease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 10^4 cell/mL in the indirect ELISA, while 10^5 cell/mL in the dot-ELISA.  相似文献   

长江中下游6月份降水的一个预报指标     

赵永平  陈永利 《海洋科学》1995,19(1):39-42

根据１９６３－１９９２年嵊山海洋站２月海气感热输送和２２ａ太阳磁周期与降水的关系，提出了一个长江中下游６月降水的综合预报指标，用此指标，对１９９３年６月长江中下游降水进行回报，结果与实况一致。  相似文献   

关于“包装产品的计量标准型一次抽检方案”的确定     

陈峥  赵维谦  李晓川 《中国海洋大学学报(自然科学版)》1989,(4)

本文给出了“包装产品的计量标准型一次抽检方案”,并通过一个实例说明本方案比一般《计量标准型一次抽检方案》更为优越。  相似文献   

胶南构造混杂岩带中糜棱岩的显微形变特征与古构造环境——来自榴辉岩围岩的构造信息     

赵广涛  张保民  韩宗珠 《中国海洋大学学报(自然科学版)》1992,(4)

以胶南构造混杂岩带中榴辉岩的围岩——套程度不同的糜棱岩化岩石为研究对象,重点研究了其显微形变的结构特征及环境意义、石英组构特征及动力学信息,并讨论其形成的古构造环境和构造机制。  相似文献   

Dynamics and control of a towed underwater vehicle system, part I: model development     

B. Buckham  M. Nahon  M. Seto  X. Zhao  C. Lambert 《Ocean Engineering》2003,30(4):82-470

Autonomous vehicles are being developed to replace the conventional, manned surface vehicles that tow mine hunting towed platforms. While a wide body of work exists that describes numerical models of towed systems, they usually include relatively simple models of the towed bodies and neglect the dynamics of the towing vehicle. For systems in which the mass of the towing vehicle is comparable to that of the towed vehicle, it becomes important to consider the dynamics of both vehicles. In this work, we describe the development of a numerical model that accurately captures the dynamics of these new mine hunting systems. We use a lumped mass approximation for the towcable and couple this model to non-linear numerical models of an autonomous surface vehicle and an actively controlled towfish. Within the dynamics models of the two vehicles, we include non-linear controllers to allow accurate maneuvering of the towed system.  相似文献   

渤海南部表层沉积的相特征   总被引：1，自引：0，他引：1  

王琦  赵其渊  杨作升  周莉  沈渭铨  周伟  李平 《中国海洋大学学报(自然科学版)》1989,(1)

通过海上调查及实验室分析,查明渤海南部表层沉积物在结构类型、矿物组合、生物组分及沉积构造等相特征方面具有明显的分区性。渤海南部存在五种沉积相:黄河水下三角洲相、陆架盆地相、海湾相、潮流浅滩相及古滨岸相。黄河水下三角洲相从河口延至水深15—20米处,沉积速率很高,沉积物富含碎屑云母及CaCo_3。陆架盆地相的沉积为粉砂质粘土,具有生物扰动构造。  相似文献   

A method for detecting the breaking of wind-generated waves in deep water   总被引：1，自引：0，他引：1  

Xu Delun  Lou Shunli  Zhao Meng  Liu Wentong 《海洋学报(英文版)》1991,10(2):281-285

The breaking of wind-generated waves is an important phenomenon in the ocean, having close relation to many aspects of the ocean, such as air-sea interaction, ocean wave dynamics, oceanic remote sensing and ocean engineering. The first problem encountered in both its theoretical study and practical measurement is how to detect the breaking of waves.  相似文献   

Cheniers in China: An overview   总被引：6，自引：0，他引：6  

Xitao Zhao 《Marine Geology》1989,90(4):311-320

In various regions along the east and southeast coast of China and on the outer shelf of the East China Sea, series of cheniers have been found and studied. Their geomorphological and sedimentological features have been described and the main conditions for their formation established.

The development of cheniers in China can be divided into three periods: 24,000-15,000 yrs B.P., 7000-5000 yrs B.P. and 5000 yrs B.P. onwards. These periods have been based on ¹⁴C datings of 80 shell samples. The chenier groups of the three periods are distributed throughout various geomorphological locations.

Using the geomorphological locations of the cheniers in combination with their age of formation, the palaeopositions of the Chinese coastline in the various periods since 24,000 yrs B.P. are described. The history of the position of the east coast of China, including the features attesting to sealevel changes during this era, is briefly discussed.  相似文献   

台湾海峡毛颚动物分布的初步研究   总被引：9，自引：0，他引：9  

蒋玫  王云龙  徐兆礼 《台湾海峡》2003,22(2):150-154

本文分析了台湾海峡毛颚动物的种类组成、数量分布特征，并就其与环境因素的关系进行了初步探讨．结果显示与台湾海峡西部、东海等邻近海域相比，本区毛颚动物具种数少，数量小，季节变化幅度较小等特点．它的数量分布状况与闽斯沿岸流、南海西南季风漂流以及黑潮支流在本区运动消长有关。  相似文献   

宁波渔业结构调整再创佳绩     

赵世养 《海洋信息》2002,(2)

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